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Incubate the membrane in the primary antibody solution for 1 to 2 hours at room temperature or overnight at 4°C with agitation. Wash the membrane three times for 3 to 5 minutes each with either water or TBS and/or PBS containing 0.05% Tween-20 (Cat No. 655204-100ML).

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17-09-2012·Second, I have always used liquid detergents because commercial powder detergents never seems to dissolve well and I just had vision of pipes clogged with chunks of hard as a rock detergent. The homemade powder is a very fine powder and for me it seems to dissolve beautifully.

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21-02-2011·hi i bought a second hand front loader and my washing was coming out clean but smelling awful after owning for over a year iv discovered the rubber is totally black due to bad hands i havent been able to move much and have tried use bleach a few times but to no avail. my hands are bad so i cant get down and really clean like i would like, i do take washing out …

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Dissolve the powder in 100 ml of methyl alcohol by warming at 50ºC for 10 minutes by shaking. Filter the solution after 24 hours Method. Smear is fixed in methanol; Stain the film with dilute dye solution (1:10) for 5 minutes; Then stain with dilute Giemsa’s stain (1:10) for 10 to 15 minutes; Wash with buffered water and dry in the air ...

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20-08-2018·A solution of 0.01-0.2% PHMB is recommended for the treatment of critical colonisation or local wound infection (Lindholm, 2010) and PHMB 0.04% is recommended for heavily colonised and clinically infected wounds – the recommended contact time is 15 minutes for all strengths of solution (Andriessen and Strohal, 2010).

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