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2001-5-1 · Pre-warm 150ml of culture medium (e.g. DMEM, RPMI) to 41°C. Place this medium in a beaker, dish, or other large-mouth container. With long forceps remove the pan of organisms from liquid nitrogen, plunge it into the culture medium, and agitate the pan a few seconds. Remove the cover from the pan as quickly as possible after thawing.

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The solution was stirred with a stir-bar until all the powder was dissolved and autoclaved at 121° C. for 20 minutes. S. papillosum extract was prepared as described in B, above. One mL of the solutions, TSB or moss-treated TSB sample, was pipetted into 3 to 5 mL polystyrene test tubes.

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The solution was stirred with a stir-bar until all the powder was dissolved and autoclaved at 121° C. for 20 minutes. S. papillosum extract was prepared as described in B, above. One mL of the solutions, TSB or moss-treated TSB sample, was pipetted into 3 to 5 mL polystyrene test tubes.

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2022-2-18 · Keep the syringe tip in the medicine. Tap the syringe with your finger to move air bubbles to the top. Then push gently on the plunger to push the air bubbles back into the vial. If you have a lot of bubbles, push the plunger to push all the medicine back into the vial. Draw medicine out again slowly and tap air bubbles out.

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2022-2-11 · Unit definition: One unit (U) is defined as the amount of enzyme that causes an increase in absorbance at 260 nm of 1.0 in 30 minutes at 37C in 50 mM Tris-HCl buffer, pH 8.0 (25C) supplemented with 5 mM MgCl2, 0.1 mg/ml BSA …

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So, for example, 150ml of sugar weighs less than 150ml of honey. How to convert grams to mL. When converting grams to milliliters, you need to remember that the gram is a unit of weight and milliliter a unit of volume. This means that to make the conversion you need to know the density of your substance (ideally in g/ml, to avoid conversion first).